Genome-wide Unbiased Identification of Double strand breaks (DSBs) Enabled by Sequencing (GUIDE-seq) is based on the efficient integration of an end-protected double-stranded oligodeoxynucleotide (dsODN) tag into the sites of nuclease-induced DSBs in live human cells. Tag-specific amplification, high-throughput sequencing, and mapping enables the identification of sites of double-strand break activity in the genome.
Tsai et al. Nat Rev Genet 2016
The GUIDE-Seq pipeline returns information regarding the location and frequency of off-target editing. This information can be used to optimize the specificity of a genome editing experiment by assessing a number of targets. For more information, see the original GUIDE-Seq publication in Nature Biotechnology (see references).
Tsai et al. Nat Biotechnology 2016
“GUIDE-Seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases,” Nature Biotechnology. 2015 Feb; 33(2): 187-197. (pdf)
"Open-source guideseq software for analysis of GUIDE-seq data," Nature Biotechnology. 2016 May; 34: 483.